Related Experiment Video
Updated: May 20, 2026

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
Published on: August 4, 2018
Controlled Activation of Auxilin-Like Protein 1 Triggers Cell Death-Associated Disease Resistance to Xanthomonas
A-Ram Jeong1, Hyeran Moon1, Oh-Kyu Kwon1
1Department of Molecular Biology, Sejong University, Seoul 05006, Republic of Korea.
Abstract:
Auxilin-like proteins (ALPs) are type III J-proteins that participate in clathrin-mediated endocytosis. In rice (Oryza sativa), an ALP, OsALP1, has been identified, but its precise role in immunity and potential for application remain largely unknown. Here, we investigated OsALP1 and its close homolog OsALP2 to elucidate their roles in rice defense. OsALP1 and OsALP2 share high sequence similarity and similar overexpression phenotypes, including spontaneous cell death and enhanced resistance to Xanthomonas oryzae pv. oryzae (Xoo). Notably, OsALP1 transcript levels were higher than those of OsALP2 in the tissues and developmental stages examined in this study, suggesting that OsALP1 is the predominant functional ALP in rice. To separate OsALP1-mediated resistance from its detrimental growth effects, we established a dexamethasone (DEX)-inducible expression system enabling temporal activation of OsALP1 in planta. DEX-induced OsALP1 expression conferred enhanced resistance to Xoo but increased susceptibility to the necrotrophic fungus Rhizoctonia solani, revealing lifestyle-dependent outcomes of immune-associated cell death. These findings provide a controllable framework for deploying OsALP1-based disease resistance while minimizing growth costs.
More Related Videos
11:50Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
Published on: October 1, 2015
06:12Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens
Published on: September 15, 2023
Related Concept Videos
Cell Signaling in Plants
The Intrinsic Apoptotic Pathway
Autophagic Cell Death
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and pro-apoptotic...
Responses to Drought and Flooding
Caspases
Gene Regulation in Microbial Communities: Quorum Sensing