Related Experiment Video
Updated: May 21, 2026
In Vitro Modeling of Fat Deposition in Metabolic Dysfunction-Associated Steatotic Liver Disease
Published on: July 19, 2024
Protocol for volumetric quantification of intrahepatic triglyceride content in adults with obesity
Alba Camacho-Cardenosa1, Antonio Clavero-Jimeno2, Alejandro López-Vázquez2
1Instituto de Investigación Biosanitaria de Granada (ibs.GRANADA), Granada, Spain; Department of Physical Education and Sports, Faculty of Sport Sciences, Sport and Health University Research Institute (iMUDS), University of Granada, Granada, Spain.
Abstract:
The study describes a protocol for the semi-automatic volumetric quantification of intrahepatic triglyceride (IHTG) levels in individuals with obesity based on the Dixon technique. Here, we describe a step-by-step procedure for image acquisition and exportation, followed by segmentation of liver regions and subsequent quantification of IHTG. This protocol is designed for application in studies with a specific focus on adults with obesity. For complete details on the use and execution of this protocol, please refer to Camacho-Cardenosa et al.1.
