Related Experiment Video
Updated: May 21, 2026

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta
Published on: December 5, 2019
The HIRA-interacting histone chaperone CABIN1 negatively regulates plant immunity mediated by immune receptor gene
Leiyun Yang1,2, Yang Liu2, Hairong Xie3
1State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Abstract:
Plant intracellular immune receptors, mostly nucleotide-binding leucine-rich repeat (NLR) proteins, are tightly regulated to maintain low expression levels under non-infection conditions and to allow induced expression by pathogens. The extensively studied NLR gene SUPPRESSOR OF NPR1, CONSTITUTIVE 1 (SNC1) is transcriptionally regulated by transcription factors, histone-modifying enzymes, and chromatin remodelers in Arabidopsis (Arabidopsis thaliana). Here, we identified a putative histone chaperone protein, CABIN1 (CALCINEURIN BINDING PROTEIN 1), as a negative regulator of SNC1-mediated plant immunity through a sensitized genetic screen. The cabin1 mutant exhibited enhanced resistance to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000. This was accompanied by elevated expression of SNC1 and many defense genes even in the absence of pathogen infection. The enhanced disease resistance in the cabin1 mutant depended on a functional SNC1 gene, and CABIN1 physically bound to the SNC1 promoter region, indicating that SNC1 directly mediates autoimmunity in cabin1. The Arabidopsis CABIN1 protein interacted with HISTONE REGULATOR A (HIRA), similarly in animals. The hira mutant also exhibited enhanced disease resistance and a higher SNC1 expression than the wild type; however, the resistance enhancement did not depend on SNC1 alone. This work identifies regulators of plant immunity and implicates histone chaperones in the regulation of immune gene expression.
More Related Videos
11:33Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Published on: October 14, 2022
11:06Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
Published on: September 20, 2017
Related Concept Videos
Regulation of Nuclear Protein Sorting
Cell Signaling in Plants
siRNA - Small Interfering RNAs
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Chromatin Immunoprecipitation- ChIP
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Experimental RNAi