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SR-B1 knockdown suppresses breast cancer cell proliferation, migration, and invasion via the PI3K/AKT pathway

Ming Li1, Yanmei Xu2, Jinwen Li3

  • 1Department of Thoracic Surgery, Shandong Public Health Clinical Center, Jinan, China.

Einstein (Sao Paulo, Brazil)
|May 19, 2026
PubMed
Abstract

Insights

Scavenger receptor class B type 1 (SR-B1) promotes breast cancer cell growth and spread. Silencing SR-B1 inhibits proliferation, migration, and invasion, offering potential therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • The function of scavenger receptor class B type 1 (SR-B1) in breast cancer is not well understood.
  • SR-B1 is a cell surface receptor implicated in cholesterol transport.

Purpose of the Study:

  • To investigate the impact of SR-B1 on breast cancer cell proliferation, migration, and invasion.
  • To explore the molecular mechanisms underlying SR-B1's role in breast cancer.

Main Methods:

  • Utilized MDA-MB-231 and MCF-7 breast cancer cell lines.
  • Employed SR-B1-specific siRNA for knockdown studies.
  • Assessed cell proliferation, migration, invasion, and apoptosis using various assays including CCK-8, colony formation, wound healing, Transwell, and flow cytometry.
  • Examined PI3K/AKT pathway activation via Western blot analysis.

Main Results:

  • SR-B1 knockdown significantly reduced breast cancer cell proliferation, migration, and invasion (p<0.05).
  • SR-B1 silencing promoted apoptosis in cancer cells.
  • Knockdown of SR-B1 decreased phosphorylation of AKT and mTOR, and downregulated downstream targets like cyclin D1 and P70.

Conclusions:

  • SR-B1 plays a crucial role in enhancing breast cancer cell proliferation and migration.
  • The PI3K/AKT signaling pathway is likely involved in mediating SR-B1's effects on breast cancer progression.

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