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Related Experiment Video

Updated: May 21, 2026

Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues

Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues

Published on: March 9, 2021

Effective Biomass Fractionation Using a Natural Acidic Polyol-Based Deep Eutectic Solvent for Sugar Recovery and

Jinwen Guo¹, Guoce Yu¹, Jianlong Wang¹

¹Laboratory of Environmental Technology, Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, China.

|May 19, 2026

Summary

This study developed an eco-friendly deep eutectic solvent pretreatment for wheat straw, achieving high sugar yields and preserving lignin integrity for efficient biomass conversion.

Keywords:

biomass lignin integrity natural deep eutectic solvent sugar recovery sustainable chemistry

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Last Updated: May 21, 2026

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Published on: June 5, 2021

Area of Science:

Biomass Conversion and Biorefining
Green Chemistry and Sustainable Processes
Biochemical Engineering

Background:

Efficient lignocellulosic biomass fractionation is critical for biorefineries.
Achieving high sugar yields while maintaining lignin integrity remains a challenge.
Environmentally benign pretreatment methods are highly sought after.

Purpose of the Study:

To investigate the efficacy of a natural deep eutectic solvent (NADES) for wheat straw pretreatment.
To optimize the NADES pretreatment process for enhanced biomass fractionation.
To evaluate the quality of extracted lignin and the recyclability of the NADES.

Main Methods:

Screening of choline chloride (ChCl)-based ternary deep eutectic solvents (DESs).
Optimization of a specific NADES (ChCl, lactic acid, glycerol) for wheat straw pretreatment.
Enzymatic hydrolysis to determine glucose yield.
Structural characterization of pretreated biomass and extracted lignin.
Recyclability tests of the NADES.

Main Results:

Optimized NADES pretreatment achieved 71.2% delignification and 55.3% xylan removal.
Resulting enzymatic hydrolysis yielded 77.2% glucose.
Pretreatment increased biomass surface area and crystallinity, disrupting lignocellulosic structure.
Extracted lignin retained high β-O-4 linkage content (45.3/100), indicating structural integrity.
Over 80% NADES recovery was achieved over five cycles with sustained glucose yields above 51.0%.

Conclusions:

A natural acidic polyol-based DES effectively fractionates lignocellulosic biomass.
The developed method offers high sugar yields and preserves lignin quality.
The NADES is recyclable and composed of eco-friendly components, supporting sustainable biorefinery strategies.