Search research articles

Search research articles

Related Experiment Video

Updated: May 21, 2026

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Second Flowing Type Lateral Flow Assay for High-Fidelity Detection.

Wenting Chang¹, Siting Fu¹, Wenyu Cao¹

¹School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.

Analytical Chemistry

|May 19, 2026

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

DNA Multiway Junction Based Lateral Flow Assay for Visualized Detection of Different Types of Targets.

Analytical chemistry·2025

Same author

Triplex DNA-Modified Gold Nanoparticles with a Tunable pH Detection Range for Multiple Colorimetric Sensing Scenarios.

Langmuir : the ACS journal of surfaces and colloids·2025

Same author

Endogenous Free-Electron-Involved Coreactant-Free Electrochemiluminescence from Nanoclusters and Its Immunoassay Application.

Analytical chemistry·2025

Same author

DNA Tetrahedron Reformative Lateral Flow Assay for Improved Detection Sensitivity and Anti-Interference.

Langmuir : the ACS journal of surfaces and colloids·2025

Same author

Controlling the Depth of Hybridization Chain Reaction by Extended Dangling Ends and Its Analytical Applications.

Analytical chemistry·2024

Same author

A DNA tetrahedron dimer for dual membrane protein logic recognition and interaction inhibition.

Chemical communications (Cambridge, England)·2024

Same journal

Machine Learning-Assisted Nanopore for Enhanced Fingerprinting Analysis of Functional Glycans.

Analytical chemistry·2026

Same journal

Correction to "Maleylpyruvic Acid-Inducible Gene Expression System and Its Application for the Development of Gentisic Acid Biosensor".

Analytical chemistry·2026

Same journal

Computer-Aided Rational Hapten Design for Broad-Spectrum Monoclonal Antibody Development against Anthraquinones and Its Application in Lateral Flow Immunoassay.

Analytical chemistry·2026

Same journal

One-Step Chemoenzymatic Labeling and Oxime-Reversible Enrichment for O-GlcNAcylation Profiling under Oxidative Stress.

Analytical chemistry·2026

Same journal

Acid/NIR Dual-Responsive Nanoplatform with AND Logic-Gated Controlled Nitric Oxide Release for Companion Theranostics of Tumors.

Analytical chemistry·2026

Same journal

Multicharged Foldable Plasma Membrane Probes for Precise Cancer Cell Discrimination and Fluorescence-Guided Surgery.

Analytical chemistry·2026

See all related articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

This study introduces a novel two-flow lateral flow assay to prevent false positives. The second flow corrects initial results, ensuring accurate detection of targets like DNA mutations and adenosine triphosphate (ATP).

Area of Science:

Biotechnology
Assay Development
Molecular Diagnostics

Background:

Lateral flow assays offer portable, instrument-free detection.
Target-sandwich assays are common but prone to false-positive signals.
Existing methods struggle with high similarity analogues and non-specific interactions.

Purpose of the Study:

To develop a high-fidelity lateral flow assay using a second flow step.
To correct false-positive signals inherent in traditional single-flow assays.
To enhance accuracy and reliability in target detection.

Main Methods:

A novel second flowing-type lateral flow assay was designed.
A second flow with displacing strands was used to validate the first flow's result.

More Related Videos

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Published on: March 22, 2012

Related Experiment Videos

Last Updated: May 21, 2026

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Published on: March 22, 2012

The assay was tested for DNA oligonucleotide mutation discrimination and adenosine triphosphate (ATP) detection.

Main Results:

The second flow successfully corrected false-positive signals.
Single base DNA mutations were accurately discriminated.
Low concentrations of ATP were differentiated from background interference.
The assay demonstrated high-fidelity detection capabilities.

Conclusions:

The second flowing-type lateral flow assay significantly improves detection accuracy.
This method provides a reliable solution for universally adopted target-sandwiched lateral flow detection.
The strategy enhances the robustness of diagnostic assays, reducing misjudgments.