Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteomic profiling of whole tissue sections in cardiac ATTR amyloidosis reveals increased extracellular matrix remodeling.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2026
Same author

Pneumococcus uses COMMD2 to alter host cellular immunity.

Nature communications·2026
Same author

Marker-Agnostic Tumor Anchoring Chimeras Enable pH-Gated Immune Engagement.

Angewandte Chemie (International ed. in English)·2026
Same author

Targeted delivery of the BCG vaccine to dendritic cells improves protective efficacy against Mycobacterium tuberculosis.

Vaccine·2026
Same author

Confirmation of monotypic immunofluorescence staining by mass spectrometry in a case of proliferative glomerulonephritis.

Histopathology·2026
Same author

Identification of DksA as a novel pro-inflammatory mediator of <i>Pseudomonas aeruginosa</i> under conditions mimicking chronic cystic fibrosis lung infection.

Virulence·2026

Related Experiment Video

Updated: May 21, 2026

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

A Platform for High-throughput and Ultrasensitive Immunopeptidomics.

Adillah Gul1, Laura Van Moortel1, Patrick Willems1

  • 1VIB Center for Medical Biotechnology, VIB, Ghent, Belgium; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium.

Molecular & Cellular Proteomics : MCP
|May 19, 2026
PubMed
Summary
This summary is machine-generated.

This study presents a semi-automated immunopeptidomics platform for high-throughput and sensitive discovery of major histocompatibility complex (MHC) peptides. The optimized workflow enables robust immunopeptide detection from low-input samples, advancing vaccine and immunotherapy research.

Keywords:
BCGListeria monocytogenesMHCantigen discoverybacillus Calmette-Guérinimmunopeptidomicsmajor histocompatibility complextimsTOF

More Related Videos

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Related Experiment Videos

Last Updated: May 21, 2026

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

  • Immunology
  • Proteomics
  • Mass Spectrometry

Background:

  • Mass spectrometry (MS)-based immunopeptidomics identifies peptides on major histocompatibility complex (MHC) molecules for vaccine and immunotherapy development.
  • Traditional workflows are low-throughput and require large cell numbers.
  • Existing methods often sacrifice sensitivity for throughput or vice versa.

Purpose of the Study:

  • To develop a semi-automated immunopeptidomics platform combining high sensitivity and high throughput.
  • To optimize conditions for isolating MHC class I and II peptides.
  • To enable robust immunopeptide detection from low-input samples.

Main Methods:

  • A 96-well positive-pressure device was used for optimized MHC class I and II peptide isolation.
  • Lysis in small volumes (100 μl) facilitated efficient MHC capture and automated processing.
  • Analysis was performed on a timsTOF SCP mass spectrometer using DDA-PASEF mode.

Main Results:

  • The platform identified over 13,500 MHC I and 6,000 MHC II peptides from 16 million cells.
  • High sensitivity was demonstrated by identifying up to 1,000 MHC I peptides from as few as 20,000 cells.
  • Known and novel bacterial immunopeptides were identified from infected macrophages.

Conclusions:

  • The optimized platform achieves high sensitivity and high throughput for immunopeptidomics.
  • It enables robust immunopeptide detection from limited sample amounts.
  • This facilitates biological discovery in applications with sample constraints.