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Updated: May 21, 2026
Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK
Published on: December 19, 2019
Engineering an azetidine-modified rhodamine probe for rapid and high-contrast imaging of ATP dynamics during
Yiteng Huang1, Le Cheng1, Shixia Tang1
1Institute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi, 530004, PR China.
Abstract:
The dynamics of adenosine triphosphate (ATP) during ischemia-reperfusion are critical determinants of cellular fate, yet tools for real-time ATP imaging remains limited. Here we developed ARho, a rhodamine-based fluorescent probe for ATP detection. ARho exhibited 627-fold fluorescence enhancement upon ATP binding, rapid response (<10 s), excellent photostability, and physiological pH compatibility. Live-cell imaging visualized ATP fluctuations induced by Ca2+ stimulation or inhibitor treatment. Furthermore, using oxygen-glucose deprivation/reperfusion (OGD/R) models, ARho successfully distinguished ATP levels in normal, ischemic, and reperfusion states in HepG2 cells, revealing distinct ATP depletion during ischemia and partial recovery upon reperfusion. Importantly, in a mouse hepatic ischemia-reperfusion model, ex vivo fluorescence imaging with ARho showed a marked decrease in ATP under ischemic conditions and a partial recovery after reperfusion, consistent with histopathological assessment. Consequently, ARho is a promising tool for monitoring ATP dynamics in both cellular and tissue models of ischemia-reperfusion injury.
