Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A review of animal-assisted therapy for older adults in Korea: effects on depression and cognitive function and implications for practice.

Journal of animal science and technology·2026
Same author

Transcriptomic Analysis of Fermented Chinese Chive Selectively Attenuating Deoxynivalenol-Induced Ovarian Toxicity in Mice.

Antioxidants (Basel, Switzerland)·2026
Same author

The Transcriptomic Landscape and Regulatory Signaling Features of Bovine Skeletal Muscle Cells Used for Cultured Meat Production.

Foods (Basel, Switzerland)·2026
Same author

Individual-dependent molecular heterogeneity in human endometrial stromal cells: a potential determinant of female fertility.

Molecular biology reports·2026
Same author

Effects of different processed forms of Panax ginseng on sperm motility and reproductive parameters in male dogs.

Journal of animal science and technology·2025
Same author

Accomplishment of canine cloning through <i>in vitro</i> matured oocytes: a pioneering milestone.

Journal of animal science and technology·2024

Related Experiment Video

Updated: May 21, 2026

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
10:50

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Published on: April 24, 2021

Engineered APP C-terminus Alters Its Native Transcript Dynamics in Differentiated SH-SY5Y-Derived Neurons.

D Chanuka M Kulatunga1, Umanthi Ranaraja1, Eun Young Kim2

  • 1Department of Animal Science and Biotechnology, Chungnam National University, Yuseong-Gu, Daejeon, Republic of Korea.

Molecular Neurobiology
|May 19, 2026
PubMed
Summary

Alzheimer's disease research reveals amyloid precursor protein (APP) fragments can regulate their own gene expression and splicing. This self-regulation offers new insights into AD pathogenesis and potential therapeutic targets.

Keywords:
AICDAlzheimer’s disease (AD)Amyloid precursor protein (APP)CTFSH-SY5YTranscript variants

More Related Videos

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
08:07

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

Published on: February 17, 2016

Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes
09:12

Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes

Published on: December 20, 2019

Related Experiment Videos

Last Updated: May 21, 2026

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
10:50

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Published on: April 24, 2021

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
08:07

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

Published on: February 17, 2016

Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes
09:12

Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes

Published on: December 20, 2019

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Alzheimer's disease (AD) pathology involves amyloid precursor protein (APP) processing.
  • APP fragments like AICD and CTF are linked to transcriptional regulation.
  • The self-regulatory role of APP fragments on APP splicing and expression is not well understood.

Purpose of the Study:

  • To investigate the self-regulatory potential of APP C-terminal fragments on endogenous APP expression and splicing.
  • To determine if APP fragments can modulate native APP transcript variants.

Main Methods:

  • Generated stable transgenic SH-SY5Y cell lines overexpressing various APP constructs.
  • Differentiated cells into mature neuron-like cells over 24 days.
  • Analyzed APP transcript variants and differential splicing patterns.

Main Results:

  • Overexpression of APP fragments altered total APP levels and specific transcript variants (APPv3, APPv11) in differentiated neurons.
  • Differential splicing patterns were confirmed via transcript fragment-size analysis.
  • APP derivatives were shown to influence transcriptional regulation and alternative splicing of native APP.

Conclusions:

  • APP fragments possess self-regulatory capabilities, modulating their own transcription and splicing.
  • This self-regulatory complexity of APP provides new insights into AD pathogenesis.
  • APP fragments may act as transcription modulators, suggesting novel therapeutic strategies for AD.