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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...
IR and UV–Vis Spectroscopy of Carboxylic Acids01:28

IR and UV–Vis Spectroscopy of Carboxylic Acids

In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency, 1710 cm−1. The C=O bond of the...
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...

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Related Experiment Video

Updated: May 21, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Deep UV Resonance Raman Spectroscopy: Applications to Membrane Proteins and Peptides.

Chanin B Tangtartharakul1, Donovan G Ruiz1, Judy E Kim2

  • 1Department of Chemistry and Biochemistry, University of California San, Diego, La Jolla, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 19, 2026
PubMed
Summary

UV resonance Raman (UVRR) spectroscopy selectively enhances signals from biomolecules. Excitation at 207 nm reveals the secondary structure of membrane-associated proteins, offering valuable molecular insights.

Keywords:
Membrane proteinsMembrane-active peptidesRaman excitation profileSecondary structureSelf-absorptionUV resonance RamanVibrational spectroscopy

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Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
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Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

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Last Updated: May 21, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

Area of Science:

  • Biochemistry
  • Spectroscopy
  • Structural Biology

Background:

  • UV resonance Raman (UVRR) spectroscopy is a vibrational spectroscopy technique.
  • It offers high selectivity for molecular insights.
  • Deep UV excitation can selectively enhance specific molecular signals.

Purpose of the Study:

  • To describe the wavelength dependence of UVRR spectra.
  • To highlight the utility of specific UV excitation wavelengths for biomolecular analysis.
  • To demonstrate the capability of 207 nm excitation for secondary structure determination.

Main Methods:

  • Utilizing UV resonance Raman (UVRR) spectroscopy.
  • Tuning the Raman excitation wavelength across the deep UV region (207–228 nm).
  • Analyzing the wavelength dependence of Raman spectra.

Main Results:

  • Selective enhancement of Raman signals from backbone and aromatic sidechains was observed.
  • The study highlights the ability of 207 nm excitation to specifically probe biomolecular structures.
  • Wavelength dependence of UVRR spectra was characterized.

Conclusions:

  • UV resonance Raman spectroscopy provides selective molecular insights.
  • 207 nm excitation is particularly effective for determining the secondary structure of membrane-associated biomolecules.
  • This technique offers a powerful tool for structural biology research.