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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...

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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in Membranes.

Viktoria Betaneli1, Jan-Hagen Krohn2,3,4, Jonas Mücksch2,5

  • 1Institute of Physiological Chemistry, Technische Universität Dresden, Dresden, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|May 19, 2026
PubMed
Summary

Fluorescence correlation spectroscopy (FCS) analyzes membrane dynamics and molecular interactions. This technique provides essential data for studying lipid-protein complexes in artificial and cellular membranes.

Keywords:
Cross-correlationDiffusionFCCSFCSFluorescence correlation spectroscopyGiant unilamellar vesiclesLipid raftsModel membranesProtein–lipid interactions

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Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)

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Last Updated: May 21, 2026

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Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)
10:59

Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)

Published on: April 6, 2012

Area of Science:

  • Biophysics
  • Membrane Biology
  • Spectroscopy

Background:

  • Fluorescence correlation spectroscopy (FCS) is a powerful technique for investigating membrane dynamics.
  • It offers insights into diffusion coefficients, concentrations, and molecular interactions of membrane components.
  • FCS is crucial for understanding protein partitioning, lipid domain identification, and lipid-protein complex formation.

Purpose of the Study:

  • To provide a comprehensive overview of FCS for membrane studies.
  • To offer practical guidelines for performing FCS experiments on artificial and cellular membranes.
  • To highlight recent methodological improvements for enhanced quantitative measurements and artifact reduction.

Main Methods:

  • Utilizes fluorescence correlation spectroscopy (FCS) principles.
  • Applies FCS to both model membrane systems and living cells.
  • Incorporates recent advancements to improve quantitative accuracy and minimize artifacts.

Main Results:

  • FCS enables detailed characterization of protein-lipid interactions within membranes.
  • The technique allows for the determination of critical membrane parameters like diffusion and concentration.
  • Recent developments enhance the reliability and precision of FCS measurements on membranes.

Conclusions:

  • FCS is an indispensable tool for studying membrane dynamics and molecular interactions.
  • The chapter equips researchers with the knowledge to conduct robust FCS studies on various membrane types.
  • Improved FCS methodologies offer more accurate and artifact-free insights into membrane biophysics.