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Updated: May 21, 2026

Visualizing Scar Development Using SCAD Assay - An Ex-situ Skin Scarring Assay
Published on: April 28, 2022
New hope for scar revision with a modified skin culture technique: a case report
W G Kleintjes1,2, T K Prinsloo3
1Department of Surgical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, South Africa.
Summary:
Cultured epidermal autografts (CEA) are seldom used in scar revision despite evidence of favourable outcomes. We report a case using a modified technique, Kleintjes Cultured Skin (KCS), for revision of childhood burn scars on the lower legs. Keratinocytes were enzymatically isolated, seeded onto the dressing, and incubated for two weeks. KCS was applied directly onto the excised scars. Scar appearance, assessed with the Vancouver Scar Scale, improved from 4 preoperatively to 2 at a follow-up of two and six years, with progressive re-pigmentation, softness, and pliability. The biopsy site also healed favourably. This case supports the potential role of KCS in cosmetic scar revision.