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Updated: May 21, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Lewis Acidic Bentonite Clays as Catalysts for 5' Deprotection of Nucleosides and Dinucleotides
Julian Marlyn1, Abhishek Arora1, Masad J Damha1
1Department of Chemistry, McGill University, Montreal, QC H3A 0B8, Canada.
Abstract:
Lewis acidic bentonite catalysts enable rapid (<10 min) and quantitative removal of 5'-O-dimethoxytrityl (DMTr) and 5'-O-(2-methoxypropyl) hydroxyl protecting groups. These catalysts selectively deprotect the 5'-OH of protected nucleosides in the presence of standard therapeutic oligonucleotide modifications. Similarly fast and quantitative deprotection of nucleotide dimers was observed, highlighting this method as an attractive option for simplified liquid phase oligonucleotide synthesis (LPOS).
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