Brevundimonas sediminis sp. nov., isolated from mangrove sediment
Shijun Pan1, Yejuan Yang1, Lirui Liu1
1Archaeal Biology Centre, Synthetic Biology Research Center, Shenzhen Key Laboratory of Marine Microbiome Engineering, Key Laboratory of Marine Microbiome Engineering of Guangdong Higher Education Institutes, Institute for Advanced Study, Shenzhen University, Shenzhen 518060, PR China.
Abstract:
Two Gram-stain-negative, facultatively anaerobic, light-yellow, rod-shaped bacterial strains, designated as FT23028T and FT23042, were isolated from mangrove sediment collected in Shenzhen, PR China. Their taxonomic positions were established using a polyphasic approach including phylogenetic, physiological, biochemical and chemotaxonomic analyses. Both strains grew optimally at 25-30 °C and pH 7.0 without NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains FT23028T and FT23042 were most closely related to Brevundimonas kwangchunensis KCTC 12380T (98.31 and 98.23%), Brevundimonas lenta KCTC 12871T (98.10 and 98.03%) and Brevundimonas viscosa CGMCC 1.10683T (97.68 and 97.61%), respectively. These two strains shared 99.93% 16S rRNA gene sequence similarity with each other. The predominant fatty acids (>10%) identified in strains FT23028T and FT23042 were C18:1 ω7c (and/or C18:1 ω6c, 48.7 and 53.2%, respectively) and C16:0 (29.9 and 26.4%, respectively). The polar lipid profiles included two unknown phosphoglycolipids, five glycolipids and three unidentified lipids. The respiratory quinone was Q-10. The total sequence lengths of strains FT23028T and FT23042 were 3,122,877 and 3,235,296 bp, with DNA G+C contents of 65.3 and 67.4 mol%, respectively. Based on phenotypic, chemotaxonomic and phylogenetic distinction, a novel species of the genus Brevundimonas is proposed, for which the name Brevundimonas sediminis sp. nov. is suggested. The type strain is FT23028T (=MCCC 1K09458T=KCTC 8783T).
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