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Related Concept Videos

Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
Centrioles and Centrosomes01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...
Meiosis II02:02

Meiosis II

Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
Meiosis II01:57

Meiosis II

Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each containing...

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Ana1/CEP295 regulates centriolar doublet-to-triplet conversion during spermatogenesis.

Zhou Chen1, Dongqing Li1, Nan Pang1

  • 1State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University , Beijing, China.

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Ana1 protein is crucial for converting microtubule doublets to triplets in Drosophila male germline cells, impacting male fertility. Its C-terminal region recruits centrobin, essential for C-tubule assembly.

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Last Updated: May 21, 2026

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09:40

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Published on: February 6, 2018

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09:41

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Structural Biology

Background:

  • Centrioles exhibit ninefold symmetry with microtubule blades, varying from singlets to triplets across species.
  • The mechanisms and biological importance of these microtubule architecture variations are not fully understood.

Purpose of the Study:

  • To investigate the role of Ana1 in regulating centriolar microtubule structure, specifically doublet-to-triplet conversion.
  • To elucidate the molecular mechanisms by which Ana1 influences C-tubule assembly.
  • To determine the biological significance of triplet microtubule integrity for male fertility.

Main Methods:

  • Utilized Drosophila male germline cells to study centriole formation and structure.
  • Investigated the function of Ana1 N-terminal and C-terminal regions in microtubule organization.
  • Examined the recruitment of centrobin by Ana1 and its role in C-tubule assembly.
  • Assessed the impact of Ana1 expression timing on male fertility.

Main Results:

  • Absence of Ana1 impairs the conversion of microtubule doublets to triplets in Drosophila male germline cells.
  • Ana1's N-terminal region promotes microtubule elongation, while the C-terminal region supports C-tubule assembly.
  • Ana1 recruits centrobin, and its recruitment is sufficient to restore C-tubule formation in Ana1-deficient cells.
  • Proper triplet microtubule structure, dependent on Ana1, is vital for male fertility.

Conclusions:

  • Ana1 is identified as a key regulator of centriolar doublet-to-triplet microtubule conversion.
  • The study reveals the mechanism of Ana1-mediated centrobin recruitment for C-tubule assembly.
  • Centriolar triplet microtubule integrity is essential for Drosophila male fertility.