Effect of HIF-1α and LDHA Blockade on Gene Expression of Tumor Immune Evasion in Myeloid Leukemia Cell Lines
Tamana Eskandari1, Mehrshad Shams2, Ali Khanari2
1Department of Immunology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Background:
Tumor cells utilize metabolic reprogramming to obtain the energy required for rapid proliferation. In this study, we blocked two key factors, hypoxia-inducible factor 1-alpha (HIF-1α) and lactate dehydrogenase (LDHA), in the metabolic pathway in acute myeloid leukemia (AML) cell lines to evaluate their effect on the expression of genes involved in tumor evasion.
Methods:
Silibinin and sodium oxamate, as HIF-1α and LDHA blockers, respectively, were used to treat K-562 and HL-60 cells. The MTT assay was used to assess cell viability, and quantitative reverse transcription PCR was used to measure the mRNA expression levels of PD-L1, CD47, and CD155.
Results:
Both K-562 and HL-60 cells showed a significant decrease in CD47 expression. Additionally, HL-60 cells indicated a significant suppression of CD155 expression. In contrast, PD-L1 expression remained unchanged after treatment.
Conclusion:
Our findings suggest that blocking signaling pathways involved in metabolic reprogramming of cancer cells could be a promising approach for modulating the expression of certain immune checkpoint ligands, warranting further investigation.