In Situ Self-Assembled Triple-Helix Molecular Switches Immobilized on 96-Well Plates: A Dual-Channel Self-Validated
Guannan Dong1, Liting Chai1, Zheyue Zhang1
1College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
Abstract:
High-throughput detection is highly significant for food safety, but traditional rapid methods usually target single analytes with a single-signal output, leading to susceptibility to interference and unsatisfactory accuracy. Herein, a high-throughput dual-signal platform was constructed by immobilizing platinum nanoparticle-modified universal triple helix molecular switches on carboxylated 96-well plates. Target recognition induces THMS structural rearrangement and dual-signal generation. Combined with smartphone quantification and MRL judgment, the biosensor showed colorimetric LODs of 0.04, 0.053, and 0.003 ng/mL and fluorescent LODs of 0.053, 0.20, and 0.075 ng/mL for OTA, acetamiprid, and Pb2+, respectively. Its reliable application in corn and wine verified its practicality, providing a promising strategy for accurate food safety POCT.
