Resveratrol at low concentrations enhances sperm-oxidative stress parameters and fertilizing-hatching potential in...

This research explored the impact of semen extenders enhanced with resveratrol (RSV) on the quality and fertility of post-thawed turkey sperm. Semen was pooled and diluted in a glucose-based extender with RSV at concentrations of 0.01, 0.1, and 1 μM, then cryopreserved. The study measured various parameters in the thawed samples, including sperm motility, motion characteristics, plasma membrane integrity, plasma membrane functionality (PMF), DNA integrity, total antioxidant capacity (TAC), glutathione peroxidase (GPx), superoxide dismutase (SOD) activity, and lipid peroxidation (LPO) levels. Fertility and hatchability were evaluated using artificial insemination with the post-thawed semen. Results indicated that extenders with 0.1 and 0.01 μM RSV significantly improved motility, motion characteristics, plasma membrane integrity, PMF, TAC, and SOD activity (p < 0.05) while reducing abnormal morphology, DNA damage, and LPO compared to other groups. Additionally, the second experiment showed higher fertility rates with 0.1 and 0.01 μM RSV (p < 0.05). In conclusion, adding 0.1 and 0.01 μM RSV enhances the quality of post-thawed turkey semen and improves sperm fertilizing capacity, making it a valuable approach to improving fertility and hatchability outcomes.