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Updated: May 22, 2026

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
Published on: May 11, 2017
HDAC3 inhibition promotes bovine oocyte maturation via the SP1-AREG pathway
Yingfan Hu1, Nuo Heng1, Yaxin Guo2
1State Key Laboratory of Animal Biotech Breeding, State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Abstract:
To investigate the effect of specifically regulating histone deacetylase 3 (HDAC3) on the in vitro maturation (IVM) of bovine oocytes and to elucidate the underlying molecular mechanisms. During IVM, COCs were treated with luteinizing hormone (LH) or different concentrations of the HDAC3-specific inhibitor HDACi 4b. Treatment with 4 μM HDACi 4b significantly promoted cumulus expansion and up-regulated the expression of expansion-related genes (HAS2, PTX3, and TNFAIP6) in cumulus cells after IVM (P < 0.05). The first polar body extrusion rate, cleavage rate, and blastocyst rate in the 4 μM HDACi 4b group were comparable to those in the LH group, with normal blastocyst quality. ELISA found no significant difference for the concentration of AREG in the culture medium between the 4 μM HDACi 4b and LH groups, but both were significantly higher than the negative control. Further exploration of the underlying mechanisms in primary granulosa cell line revealed that HDACi 4b inhibited the expression of HDAC3 protein in a time-dependent manner and concurrently up-regulating H3K14ac and AREG levels (P < 0.05). While inhibition of the transcription factor SP1 significantly attenuated the HDACi 4b-induced up-regulation of AREG (P < 0.05). The HDAC3-H3K14ac-SP1-AREG signaling pathway plays a critical regulatory role in the maturation of bovine oocytes. Our work profiles a novel and stable strategy for bovine oocyte IVM.
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