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The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...

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Related Experiment Video

Updated: May 22, 2026

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

Autoinhibitory feedback preserves intestinal stem cell maintenance and fate commitment.

Siamak Redhai^1,2,3, Nick Hirschmüller^4,5, Tianyu Wang^6,7,8

¹German Cancer Research Center (DKFZ), Division Signaling and Functional Genomics, Heidelberg, Germany. siamak.redhai@dkfz.de.

The EMBO Journal

|May 20, 2026

Summary

This summary is machine-generated.

A new study reveals Chronophage (Cph) acts as a self-limiting program in Drosophila midgut intestinal stem cells (ISCs). This transcription factor balances ISC renewal and differentiation into enteroendocrine (EE) cells, preventing cell death and preserving tissue function.

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Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

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Last Updated: May 22, 2026

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

Published on: April 1, 2021

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Published on: July 9, 2021

Area of Science:

Developmental Biology
Stem Cell Biology
Genetics

Background:

Intestinal stem cells (ISCs) are crucial for gut epithelium renewal.
Mechanisms linking ISC renewal and lineage commitment are not fully understood.

Purpose of the Study:

To identify regulatory mechanisms governing ISC maintenance and differentiation.
To characterize the role of Chronophage (Cph) in Drosophila midgut stem cell fate.

Main Methods:

Genetic analysis in Drosophila midgut.
Single-cell transcriptomic profiling.
Identification of transcription factor Chronophage (Cph) and its targets.

Main Results:

Chronophage (Cph) mediates a self-limiting transcriptional program for ISC renewal and enteroendocrine (EE) cell differentiation.
Cph expression is induced by scute and is essential for ISC reprogramming and lifespan.
Cph directly represses its own expression, creating an autoinhibitory feedback loop that prevents ISC death.

Conclusions:

A novel regulatory mechanism balances stem cell maintenance and differentiation through Cph.
Autoinhibitory feedback by Cph preserves ISC function and prevents autophagosome accumulation.
Findings offer insights into principles governing regenerating tissues.