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Updated: May 22, 2026

A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

Published on: December 16, 2022

Fetal Mouse Heart Clearing, Imaging, and 3D Reconstruction.

Hamna Ammar¹, Paul Delgado-Olguín^2,3,4

¹Translational Medicine, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, Toronto, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|May 20, 2026

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Three-dimensional imaging of cleared embryonic hearts using light sheet fluorescence microscopy offers a more efficient method for detecting congenital heart defects (CHDs) in mouse models. This advanced technique improves visualization and reduces the time and labor associated with traditional histological sectioning.

Area of Science:

Developmental biology
Medical imaging
Cardiovascular research

Background:

Traditional histological sectioning is standard for examining congenital heart defects (CHDs) in mouse models.
This method is time-consuming, labor-intensive, and may miss subtle defects due to serial sectioning requirements.

Purpose of the Study:

To present a more efficient and comprehensive method for detecting CHDs in mouse embryos.
To highlight the advantages of 3D imaging over traditional histological techniques.

Main Methods:

Utilized three-dimensional imaging of cleared embryonic hearts.
Employed light sheet fluorescence microscopy for rapid z-stack acquisition.
Reconstructed high-resolution 3D models from acquired data.

Keywords:

3D reconstruction Congenital heart defects Embryonic heart Light sheet microscopy Tissue clearing

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Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound (30/45MHZ) System

Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound (30/45MHZ) System

Published on: May 5, 2018

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Published on: October 7, 2016

Related Experiment Videos

Last Updated: May 22, 2026

A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

Published on: December 16, 2022

Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound (30/45MHZ) System

Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound (30/45MHZ) System

Published on: May 5, 2018

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Published on: October 7, 2016

Main Results:

The 3D imaging technique significantly improves the detection of CHDs in mouse fetuses.
This approach enables rapid acquisition of extensive data without manual sectioning.
Visualization of subtle defects is enhanced compared to traditional methods.

Conclusions:

Light sheet fluorescence microscopy provides a superior alternative to traditional histology for CHD analysis in mouse models.
This technique offers enhanced efficiency, comprehensiveness, and detection accuracy for embryonic heart development studies.