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Related Experiment Video

Updated: May 22, 2026

Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts
09:29

Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts

Published on: March 22, 2017

Embryonic Mouse Cardiac Fibroblast Isolation.

Enrique Mendez-Bolaina1, Elena de la Cruz Herrera-Cogco2, Israel Ramírez-Sánchez3

  • 1Facultad de Ciencias Químicas, Universidad Veracruzana, Orizaba, Veracruz, México.

Methods in Molecular Biology (Clifton, N.J.)
|May 20, 2026
PubMed
Summary

This study presents a method for culturing mouse embryonic cardiac fibroblasts (MEFs). This technique ensures high cell yield, quality, and viability for reliable cardiac research.

Keywords:
Cardiac fibroblastsCell cultureEmbryonic miceEnzymatic digestionHeartIsolation

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Isolation of Embryonic Ventricular Endothelial Cells
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Related Experiment Videos

Last Updated: May 22, 2026

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Isolation of Embryonic Ventricular Endothelial Cells
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Published on: July 20, 2013

Area of Science:

  • Cardiovascular Biology
  • Cell Biology
  • Developmental Biology

Background:

  • Primary mouse cardiac fibroblasts (FBs) are crucial in vitro models for studying heart disease mechanisms and therapeutic targets.
  • Fibroblasts in culture rapidly lose proliferative capacity and undergo senescence postnatally.
  • This limits their utility for long-term or detailed experimental analysis.

Purpose of the Study:

  • To establish a reliable method for primary culture of mouse embryonic cardiac fibroblasts (MEFs).
  • To ensure high cell yield, quality, and viability for accurate experimental outcomes.
  • To provide a robust model for studying cardiac biology and pathology.

Main Methods:

  • Isolation of cardiac fibroblasts from embryonic day 16 (E16) CD-1 mouse embryos.
  • Optimization of cell culture conditions to maintain fibroblast characteristics.
  • Assessment of cell yield, viability, and proliferative capacity.

Main Results:

  • The described method yields a high number of viable mouse embryonic cardiac fibroblasts.
  • Primary MEF cultures exhibit sustained proliferative capacity compared to postnatal FBs.
  • The cells maintain their differentiated phenotype suitable for experimental manipulation.

Conclusions:

  • This method provides a reliable source of high-quality mouse embryonic cardiac fibroblasts.
  • These MEFs serve as an improved in vitro model for cardiac research.
  • The technique facilitates the study of cardiac pathologies and the identification of therapeutic targets.