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Updated: May 22, 2026

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
09:45

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

Optimized tRNA processing and TREX2-SpCas9 fusion enable high-efficiency multiplex genome editing in plants

Ziyan Xu1, Shengqun Qiu2, Yuchong Tan1

  • 1State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Plant Communications
|May 21, 2026
PubMed
Summary

This study introduces an optimized CRISPR system for multiplex genome editing in rice, enhancing efficiency and enabling simultaneous modification of multiple genes. The new system uses a TREX2-SpCas9 fusion and improved tRNA-based guide RNA processing for advanced crop engineering.

Keywords:
CRISPR–SpCas9OsCPKTREX2multiplex gene editingricetRNA

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