Self-Assembly of Stimuli-Responsive Peptide Enhances Therapeutics by Specifically Disrupting Hepatocellular Carcinoma...

Lysosome sequestration or drug-triggered autophagic flux curtails antitumor drug potency in hepatocellular carcinoma (HCC) and can potentially be reversed with tumor cell-specific lysosomal disruption. Here, we demonstrate that a chimeric peptide (RS-FS), consisting of HCC-targeting RS and nanostructure-forming motifs (FS), self-assembles into nanospheres at neutral pH and transforms into nanofibers under acidic and reductive conditions. These nanofibers specifically localize to tumors and disrupt tumor cell lysosomes, thus enhancing doxorubicin's activity in human HCC cells in vitro and orthotopic HCC mice in vivo after RS-FS-doxorubicin treatment. Importantly, intravenous RS-FS potentiated oral Lenvatinib's antitumor activity up to 61-fold, and eradicated tumors in orthotopic HCC mice via HCC cell-specific lysosome disruption. Potent antitumor effects were also achieved with intravenous RS-FS and oral Epimedium brevicornu Maxim. -derived extracellular vesicles in orthotopic HCC mice, with markedly reduced tumor growth and increased cytotoxic T infiltration, in which RS-FS-mediated lysosome disruption promoted drug release and autophagic flux blockade. Our study demonstrates that RS-FS self-assembles into nanospheres or nanofibers in response to stimuli and enables tumor cell-specific lysosome disruption, resulting in enhanced drug release, autophagic flux blockade, and antitumor activities of diverse therapeutics in HCC mice, and thus provides a generalizable peptide adjuvant for sensitizing HCC-targeted therapeutics.