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  3. Enzymatic Methods For Assembling And Modifying Hydrogel Biomaterials.
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  3. Enzymatic Methods For Assembling And Modifying Hydrogel Biomaterials.

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Enzymatic Methods for Assembling and Modifying Hydrogel Biomaterials.

Irina Kopyeva1, Cole A DeForest1,2,3,4,5,6

  • 1Department of Bioengineering, University of Washington, Seattle, WA 98105, USA.

Regenerative Engineering and Translational Medicine
|May 21, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

Enzymes enable efficient hydrogel synthesis and modification for biomedical applications. This review explores various enzyme classes and their substrates, highlighting benefits and limitations for advanced biomaterial development.

Keywords:
EnzymesHydrogelStimuli-responsive

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Area of Science:

  • Biomaterials Science
  • Biochemistry
  • Chemical Engineering

Background:

  • Enzymes, biological catalysts, are crucial for hydrogel synthesis and modification.
  • Hydrogels are water-swollen polymeric networks with diverse biomedical applications.
  • Enzymatic techniques are valuable for mimicking tissue environments in lab assays.

Purpose of the Study:

  • To review the literature on enzymatic hydrogel synthesis and modification.
  • To examine various enzyme classes and their substrates used in hydrogel applications.
  • To discuss the benefits and limitations of enzymatic approaches for biomaterial development.

Main Methods:

  • Literature review conducted using Google Scholar, PubMed, and Web of Science.
  • Analysis of enzyme classes including transglutaminases, oxidoreductases, transpeptidases, and proteinases.
  • Evaluation of substrate incorporation into peptide precursors and synthetic polymers.

    • Main Results:

    • Diverse enzyme classes facilitate hydrogel assembly and disassembly.
    • Enzymatic substrates can be integrated into peptide precursors or polymer backbones.
    • Benefits and limitations were assessed based on ease of use, synthesis, accessibility, and cost.

    Conclusions:

    • Enzymes are versatile tools for modifying natural and synthetic biomaterials.
    • Leveraging "biologically invisible" enzymes like sortases is key for advanced, stimuli-responsive platforms.
    • Enzymatic methods should be chosen carefully to avoid interference with native biological processes.