Related Experiment Video
Updated: May 22, 2026
Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins
Published on: November 18, 2016
Increased metallopeptidase activity, compromised endothelial integrity and impaired pathogen recognition revealed by
Lucienne Tritten1, Annageldi Tayyrov1, Lennart Opitz2
1Institute of Parasitology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 266a, Zürich, 8057, Switzerland.
Abstract:
Dogs infected with the cardiopulmonary nematode Angiostrongylus vasorum are at high risk to die because of the associated bleeding pathologies, which remain poorly understood. Given their central role in immune surveillance and signaling, we analyzed transcriptomic profiles from (i) A. vasorum-infected dogs presenting with hypocoagulability (Infhypo; n = 5), (ii) infected dogs with normal coagulation values (Infnorm; n = 5), and (iii) uninfected controls (UC; n = 12). Alongside classical hematology and coagulation parameters, including rotational thromboelastometry (ROTEM), we generated a global transcriptomic profile of circulating blood cells, enabling the identification of differentially expressed genes following infection. Fibrinogen levels were significantly lower in Infhypo than in Infnorm dogs. All Infhypo dogs showed out-of-range prothrombin times. Matrix metalloprotease transcripts were strongly upregulated among infected dogs, regardless of their coagulation status, which might amplify vascular disruption and inflammation. Analysis of downregulated transcripts in infected dogs compared to UC indicated a loss of endothelial integrity, especially at the level of the lungs, and of inflammatory processes contributing to fibrinolysis and coagulopathies. Endothelial injury is a known trigger of disseminated intravascular coagulation (DIC) and consumption of coagulation factors, which could explain the observed coagulation disorders induced by A. vasorum infection. The multifaceted alterations observed in hematologic and coagulation parameters, ROTEM, and transcriptomic profiles of A. vasorum-infected dogs point to a dynamic and evolving disruption of hemostatic balance. While some animals show signs of early compensatory hypercoagulability, others seem to progress toward a state consistent with DIC, marked by factor consumption, endothelial dysfunction, and impaired fibrinogen-dependent clotting.
