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Updated: May 23, 2026

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically
Published on: September 9, 2015
Protocol for determining minimum inhibitory concentrations of essential oils against bacterial pathogens using broth
Maura Di Vito1, Melinda Mariotti1, Mattia Di Mercurio1
1Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy.
Abstract:
Essential oils (EOs) are widely investigated for antimicrobial activity, yet minimum inhibitory concentrations (MICs) lack cross-study comparability due to the absence of a harmonized reference assay. Here, we present a protocol to determine MICs of EOs against aerobic bacterial pathogens using a broth microdilution technique. We describe steps for incorporating EO dispersion using Tween 80 and preparing a standardized bacterial inoculum. We detail procedures for establishing two-fold dilution series in 96-well plates and visually determining the MIC endpoint.
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