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Structure of exopolysaccharides produced by two strains of Lactiplantibacilluspentosus
Evgeny Vinogradov1, Irina Sadovskaya2, Emmanuel Maes3
1National Research Council Canada, Ottawa, ON, K1A 0R6, Canada.
Abstract:
Exopolysaccharides (EPS) were purified from two Lactiplantibacillus pentosus spontaneous EPS-overproducing mutants, derived from two strains originating from fermented vegetal products. Their chemical structure was established by methylation analysis, 2D NMR spectroscopy, and mass spectrometry. The first EPS isolated from strain VES7988 has an original structure made of O-acetylated sialyl-lactose repeating units. The second EPS isolated from strain VES8020 is an acidic polysaccharide made of linear nonasaccharide repeating units linked by phosphodiester bonds. The third EPS also isolated from VES8020 is a neutral polysaccharide made of branched pentasaccharide repeating units.
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