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Updated: May 23, 2026

Profiling the Bacterial Community of Fermenting Traminette Grapes during Wine Production using Metagenomic Amplicon Sequencing
Published on: December 1, 2023
Biokinetic profiling of nutritional value and bioactivity in grape and apple pomace during microbial proliferation
Richard Q Mensah1, Yinling Gao1, Kameron Dunn1
1Centre of Agriculture and the Bioeconomy, Queensland University of Technology, 2 George Street, Brisbane QLD 4000, Australia; School of Mechanical Medical, and Process Engineering, Faculty of Engineering, Queensland University of Technology, 2 George Street, Brisbane QLD 4000, Australia.
Abstract:
Globalisation coupled with dietary shifts, have exponentially increased agrifood waste (AFW) generation. Shiraz (SGP), chardonnay (CGP), and apple (AP) pomaces represent agrifood waste with minimal carbon abatement value. These residues contain components with significant biological activities. However, high moisture contents (50 - 85 %) make agrifood waste susceptible to microbial growth. Therefore, clarity of correlation between passive microbial growth and bioactivity in agrifood waste is essential for developing biorefinery systems. This study examined how passive microbial proliferation impacts polyphenols, pectin, and antioxidant activity in agrifood waste. Increased microbial proliferation corresponded to decrease in polyphenols and antioxidant capacity, but not pectin yield. This could be associated with the presence of Saccharomyces cerevisiae and Pichia manshurica. Pectin yield of grape and apple pomace increased after a week of microbial action. The reduction in polyphenol and antioxidant activity of agrifood waste was fit into different kinetic models. The kinetics of bioactivity reduction best followed first-order kinetics, with CGP components (-0.0055 h-1) decreasing faster than SGP (-0.0030 h-1), and AP (-0.0008 h-1). The kinetics observed in this study indicate that bioactivity reduction rate is proportional to the levels of residual phenolics in these food residues. Overall, the offsetting increase in pectin yield during microbial proliferation highlights a complementary valorisation pathway, underscoring the value of characterising passive microbial dynamics in agrifood waste biorefinery development.
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