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Updated: May 23, 2026

Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples
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CONFIRMATION OF SARCOCYSTIS INFECTIONS IN TONGUES OF FLORIDA PANTHERS (PUMA CONCOLOR CORYI).

J P Dubey, Aditya Gupta, Mason V Reichard

    The Journal of Parasitology
    |May 21, 2026
    PubMed
    Summary
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    EFFECTS OF AVIAN MALARIA IN A POPULATION OF CERULEAN WARBLER (SETOPHAGA CERULEA) WITH FIRST DOCUMENTATION OF HAEMOSPORIDIAN LINEAGES INFECTING THE SPECIES.

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    Sarcocystis parasites were identified in Florida panthers, with one species confirmed as Sarcocystis felis. Further research is needed to identify a second, distinct Sarcocystis type found in these wild felids.

    Area of Science:

    • Veterinary Parasitology
    • Wildlife Disease Ecology
    • Molecular Biology

    Background:

    • Sarcocystis species are protozoan parasites with complex life cycles involving wild and domestic animals.
    • Florida panthers (Puma concolor coryi) are an endangered subspecies facing various health challenges.
    • Understanding parasite prevalence in apex predators is crucial for ecosystem health.

    Purpose of the Study:

    • To determine the prevalence and identify Sarcocystis species in Florida panther tongues.
    • To characterize the morphology and molecular genetics of detected Sarcocystis.
    • To assess the potential impact of Sarcocystis on panther health.

    Main Methods:

    • Necropsy and light microscopy of tongue tissues from Florida panthers.
    • Morphological analysis of sarcocysts, including sarcocyst wall structures.
    Keywords:
    Florida panther (Puma concolor coryi)PhylogenySarcocystisSarcocystis felisSarcocystsTongue

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  • Molecular identification using the ITS1 gene sequencing.

    • Main Results:

    • Sarcocysts were detected in 42.6% (46/108) of Florida panthers examined.
    • Two distinct morphological types of sarcocysts were observed: one with serrated walls and one with smooth walls.
    • Molecular analysis confirmed the serrated type as Sarcocystis felis.

    Conclusions:

    • Sarcocystis felis is present in Florida panthers.
    • An unidentified Sarcocystis species with smooth-walled sarcocysts also infects this population.
    • Further molecular and morphological studies are required to fully characterize the novel Sarcocystis species.