Biocatalytic membrane for membrane inlet mass spectrometry

Area of Science:

• Biotechnology
• Analytical Chemistry
• Biomaterials

Background:

• Enzyme immobilization is crucial for developing biosensors.
• Polydimethylsiloxane (PDMS) membranes are widely used in analytical devices.
• Eggshell membranes offer a biocompatible and functionalizable platform.

Purpose of the Study:

• To develop a novel method for immobilizing enzymes on a PDMS membrane probe for membrane inlet mass spectrometry (MIMS).
• To utilize eggshell membranes as a support for enzyme immobilization via covalent linkage.
• To determine glucose concentrations in complex matrices using the developed enzyme-immobilized probe.

Main Methods:

• Enzyme immobilization onto an eggshell membrane using glutaraldehyde cross-linking.
• Development of a membrane probe for MIMS.
• Analysis of glucose in various complex samples (wine, juice, milk, plasma).
• Characterization of the immobilized enzyme membrane using scanning electron microscopy (SEM).

Main Results:

• The developed probe demonstrated a linear response range of 50 to 1000 µmol·L⁻¹ for glucose.
• The response time was approximately 120 seconds.
• Sensitivity was determined to be 67.8 µmol·L⁻¹ decrease in dioxygen concentration per 1 mmol·L⁻¹ of glucose.
• The method showed comparable results to a glucose oxidase amperometric biosensor.

Conclusions:

• Eggshell membranes provide an effective matrix for enzyme immobilization for MIMS applications.
• The developed probe is suitable for quantifying glucose in diverse and complex biological and food samples.
• The method offers a stable and sensitive approach for enzymatic analysis.