Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Inflammatory Bowel Disease II: Ulcerative Colitis

Inflammatory Bowel Disease II: Ulcerative Colitis

Ulcerative colitis is a chronic inflammatory disorder of the colon characterized by continuous mucosal inflammation that typically begins in the rectum and extends proximally in a uniform pattern. Its pathogenesis involves a complex interplay of genetic predisposition, immune dysregulation, and environmental influences. These factors converge to impair the colon’s epithelial defenses and promote an exaggerated inflammatory response against luminal contents.Breakdown of the Mucosal BarrierA...

Renewal of Intestinal Stem Cells

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Lipidomics-Based Investigation of the Effects of Ginsenoside FI on Free Fatty Acid-Induced Metabolism in HepG2 Cells.

Pharmaceuticals (Basel, Switzerland)·2026

Same author

Relationship between structural/conformational differences and gel properties in pectins extracted under different acidic conditions.

International journal of biological macromolecules·2026

Same author

Modulating limonin release via pectin fine structure in Ca<sup>2+</sup>-citrus pectin emulsion gels.

Food research international (Ottawa, Ont.)·2025

Same author

Citrus endogenous components as prebiotics: Advances in extraction, digestion, mechanisms, and delivery.

Food research international (Ottawa, Ont.)·2025

Same author

Physicochemical and rheological properties of konjac glucomannan and its mixed gels with κ-carrageenan: Effect of deacetylation by L-arginine.

Food chemistry·2025

Same author

Preparation of citrus fiber from lemon peel residue: Effects of structure and endogenous pectin components on emulsifying properties of citrus fiber.

International journal of biological macromolecules·2024

Search research articles

Related Experiment Video

Updated: May 23, 2026

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Published on: January 5, 2017

Differential Modulation of Colonic Inflammaging by Limonin Loaded in Pectin Emulsion Gels with Engineered Fine

Wei-Yun Zhang¹, Jin-Song Liao², Jun-Ru Qi¹

¹Research and Development Center of Food Proteins, School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, PR China.

Journal of Agricultural and Food Chemistry

|May 22, 2026

Summary

Pectin-based emulsion gels (EGs) loaded with limonin show distinct anti-inflammatory effects on colonic inflammaging in aged mice. Structural variations in EGs enable targeted interventions for gut health.

Keywords:

colonic inflammaging emulsion gel limonin pectin

More Related Videos

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants

Published on: May 1, 2013

Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Published on: June 2, 2023

Related Experiment Videos

Last Updated: May 23, 2026

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Published on: January 5, 2017

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants

Published on: May 1, 2013

Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Published on: June 2, 2023

Area of Science:

Biomaterials Science
Gastroenterology
Immunology

Background:

Limonin bioavailability is enhanced by emulsion gel (EG) systems.
The specific targets of limonin-loaded EGs in colonic inflammaging, based on pectin structure, are not well understood.

Purpose of the Study:

To investigate the differential anti-inflammatory effects of pectin-based EGs with varying structures on colonic inflammaging.
To elucidate the structure-dependent mechanisms by which these EGs modulate gut inflammation.

Main Methods:

Engineered three distinct pectin-based EGs by altering esterification degree and RG-I side-chain ratio.
Administered EGs to d-galactose-induced aged mice.
Assessed anti-inflammatory effects, barrier function, pyroptosis inhibition, gut microbiota composition, and cytokine expression.

Main Results:

All EGs showed superior anti-inflammatory effects compared to free limonin.
One EG enhanced chemical and mechanical gut barriers.
Another EG significantly inhibited pyroptosis and ameliorated colonic immune barrier damage.
A third EG modulated the Erysipelotrichaceae family and suppressed inflammatory cytokines.

Conclusions:

Structural variations in pectin-based EGs lead to distinct mechanisms for regulating colonic inflammaging.
Tailoring EG structure allows for targeted interventions against gut inflammation and immune barrier damage.