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Curcumin nanoparticles combined with narlumosbart regulate wingless-type MMTV integration site (wnt)/β-catenin
1Department of Orthopaedic Surgery (hand and foot trauma), People's Hospital of Dongxihu, Wuhan, Hubei, China.
Background:
Narlumosbart is a key agent for modulating bone cell function. Given the potential of curcumin nanoparticles to inhibit cell growth when applied alone.
Objectives:
This study aims to investigate the role of their combination with narlumosbart in bone trauma repair and cell cycle regulation.
Methods:
Curcumin nanoparticles were fabricated and applied in combination with narlumosbart to treat MC3T3-E1 cells. The effects of this combination on osteoblast differentiation and proliferation were then assessed through immunofluorescence, protein expression analysis and ALP activity assays.
Results:
The combined use of curcumin nanoparticles and narlumosbart at 4 µM significantly enhanced inhibitory activity, indicating a synergistic therapeutic effect. Furthermore, immunohistochemistry revealed a marked increase in β-catenin expression and activation of the Wnt/β-catenin signaling pathway in the combination group. In contrast, while ALP staining confirmed elevated ALP activity, immunofluorescence detection showed no significant change in cell proliferation.
Conclusion:
Loaded within narlumosbart, curcumin nanoparticles can precisely modulate the Wnt/β-catenin pathway. This process specifically induces osteogenic differentiation without impacting proliferation, thereby directing cellular processes towards bone formation.
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