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Updated: May 23, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
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Characterisation of the Novel HLA-DQB1*06:03:58 Allele by Next-Generation Sequencing

Mohamed Amine Hmida1, Grégory Gatouillat1, Grégory Salez1

  • 1Laboratoire d'immunologie, CHU Reims, Reims, France.

HLA
|May 22, 2026
PubMed

Keywords:
HLA‐DQB1*06:03:58HLAnext‐generation sequencingnovel allele

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Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.