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Au@Pt core-shell nanoparticle-based lateral flow immunoassay for rapid detection of Porphyromonas gingivalis type II
Junheng Chen1,2, Zhenjie Lu3, Xiaojun Wang3
1The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, People's Republic of China. biowyl@126.com.
Abstract:
Porphyromonas gingivalis is a principal etiological agent of periodontal inflammation, with strains expressing type II and IV FimA fimbrial proteins exhibiting increased virulence. In this study, we developed a rapid and sensitive immunochromatographic assay for the detection of type II and IV FimA proteins, utilizing Au@Pt core-shell nanoparticles for catalytic signal amplification. Following optimization, the optimal antibody labeling quantities were determined to be 19.25 µg for FimA II and 22 µg for FimA IV, with a capture antibody concentration of 1.5 mg mL-1 for both test strips. Visual detection was achieved within 14 minutes. The limits of detection were established at 25 ng mL-1 for FimA II and 12.5 ng mL-1 for FimA IV prior to catalysis, with an approximate eight-fold decrease following catalytic amplification. The assay demonstrated excellent specificity and high concordance with PCR results in 112 clinical samples (AUCs: 0.9075 and 0.9544). This method offers a rapid and reliable analytical tool for the screening of high-risk P. gingivalis infections and has the potential to be adapted for the detection of other systemic diseases associated with P. gingivalis.
Insights
A new immunochromatographic assay rapidly detects virulent Porphyromonas gingivalis strains. This tool utilizes advanced nanoparticles for sensitive detection of FimA proteins, aiding in early diagnosis of periodontal disease.
Area of Science:
- Biotechnology
- Immunology
- Nanotechnology
Background:
- Porphyromonas gingivalis is a key cause of periodontal inflammation.
- Strains with type II and IV FimA fimbrial proteins show higher virulence.
Purpose of the Study:
- To develop a rapid and sensitive immunochromatographic assay for detecting type II and IV FimA proteins.
- To utilize Au@Pt core-shell nanoparticles for enhanced signal amplification.
Main Methods:
- Optimization of antibody labeling and capture antibody concentrations.
- Development of a visual detection assay with a 14-minute timeframe.
- Determination of limits of detection before and after catalytic amplification.
Main Results:
- Optimal antibody labeling: 19.25 µg for FimA II, 22 µg for FimA IV.
- Capture antibody concentration: 1.5 mg mL⁻¹ for both.
- Limits of detection: 25 ng mL⁻¹ (FimA II) and 12.5 ng mL⁻¹ (FimA IV) pre-catalysis, with ~8x improvement post-catalysis.
- High specificity and concordance with PCR in 112 clinical samples (AUCs: 0.9075, 0.9544).
Conclusions:
- The developed assay is a rapid and reliable tool for screening high-risk Porphyromonas gingivalis infections.
- This method has potential for adaptation to detect other systemic diseases linked to P. gingivalis.
