Related Experiment Video
Updated: May 24, 2026
07:52
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
Published on: November 2, 2020
Protocol for an in vivo CRISPR screen for germinal center B cells in mice using ecotropic retrovirus
Jun P Hong1, Michel C Nussenzweig2
1Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
STAR Protocols
|May 22, 2026
Abstract:
The lack of an in vitro model that recapitulates germinal center (GC) B cell biology necessitates the use of animal models for genetic studies. Here, we present a protocol for an in vivo CRISPR screen for GC B cells in mice using an ecotropic retrovirus. We describe steps for constructing a single guide (sgRNA) library and performing a genetic screen in a mouse model of protein immunization, including procedures for sequencing and data analysis.
