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Related Experiment Video

Updated: May 24, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

High-fidelity molecular decoding through tile-guided in situ self-assembly.

Xiaolin Hu1, Xinlin Guo1, Jie Xie1

  • 1Department of Laboratory Medicine, Chongqing Center for Clinical Laboratory, Chongqing Academy of Medical Sciences, Chongqing General Hospital, School of Medicine, Chongqing University, Chongqing 401147, China.

Science Advances
|May 22, 2026
PubMed
Summary

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We developed a DNA tile-based strategy for robust, amplified imaging of multiple cancer biomarkers inside cells and in mice. This method enhances signal detection and improves cancer classification through precise biomarker profiling.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cancer Research

Background:

  • Intracellular biomarker profiling is key for understanding cancer development and cell types.
  • Existing methods struggle with high background noise and unstable signals.

Purpose of the Study:

  • To introduce a novel DNA tile-driven in vivo logical encoding self-assembly (TILES) strategy.
  • To achieve robust, signal-amplified imaging of multiple intracellular biomarkers in cancer cells and in vivo.

Main Methods:

  • Utilized AND-gated logic operations for dual biomarker recognition.
  • Initiated intracellular self-assembly of DNA scaffolds forming nanotube-like signals.
  • Assessed nuclease resistance and signal amplification of the DNA scaffolds.

More Related Videos

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

Related Experiment Videos

Last Updated: May 24, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

Main Results:

  • The TILES assay demonstrated robust and amplified imaging of multiple biomarkers.
  • DNA scaffolds showed over sevenfold enhanced nuclease resistance compared to dsDNA probes.
  • The structural output improved visualization and classification of diverse cancer types.

Conclusions:

  • The TILES strategy offers a high-fidelity platform for multiplexed intracellular biomarker profiling.
  • This approach enables dynamic gene regulatory network analysis in living organisms.
  • The method enhances cancer cell classification and understanding of tumorigenesis.