Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

Overview
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
Lipids as Anchors01:32

Lipids as Anchors

In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains the...
Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin, triggering...
The Early Endosome: Endocytosis of Transferrin01:28

The Early Endosome: Endocytosis of Transferrin

Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A proximity-labeling map of PI5P4K phosphoinositide kinases interaction networks.

The Journal of biological chemistry·2026
Same author

Pharmacological modulation of lipid signaling.

Methods in enzymology·2026
Same author

A Legacy of Mentorship: A Tribute to Lewis Cantley.

Cancer discovery·2026
Same author

Boosting immunotherapy by restoring cryptic APC function.

Cell research·2026
Same author

Cholesterol efflux protein, ABCA1, supports anticancer functions of myeloid immune cells.

Science advances·2026
Same author

EGFR activation requires cholesterol interaction at the inner leaflet of the plasma membrane.

Science advances·2025

Related Experiment Video

Updated: May 24, 2026

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
08:49

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

Noncanonical PI(4,5)P2 coordinates lysosome positioning through cholesterol trafficking.

Ryan M Loughran1, Gurpreet K Arora1, Jiachen Sun2

  • 1Cancer Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA.

Science Advances
|May 22, 2026
PubMed
Summary

Targeting cholesterol metabolism is key for p53-deficient cancers. PI5P4Ks regulate lysosomal cholesterol transport and mTOR signaling, offering new therapeutic avenues beyond statins.

More Related Videos

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
07:26

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
08:15

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Published on: May 22, 2017

Related Experiment Videos

Last Updated: May 24, 2026

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
08:49

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
07:26

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
08:15

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Published on: May 22, 2017

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • p53-deficient cancers exhibit dysregulated cholesterol biosynthesis via SREBP2 pathways.
  • Statins targeting cholesterol synthesis show promise but face acquired resistance.
  • Comprehensive strategies require inhibiting both cholesterol synthesis and intracellular transport.

Purpose of the Study:

  • Investigate the role of Phosphatidylinositol-5-phosphate 4-kinases (PI5P4Ks) in cholesterol homeostasis.
  • Elucidate the upstream mechanisms linking PI5P4Ks to mTOR signaling and tumor proliferation in p53-deficient cancers.

Main Methods:

  • Enzyme kinetics of PI5P4Ks in converting PI(5)P to PI(4,5)P2.
  • Analysis of lysosomal cholesterol transport and positioning.
  • Assessment of mTOR pathway signaling and tumor growth.

Main Results:

  • PI5P4Ks are crucial for lysosomal cholesterol transport.
  • PI5P4Ks regulate lysosome positioning within cells.
  • PI5P4Ks sustain growth signaling through the mechanistic target of rapamycin (mTOR) pathway.

Conclusions:

  • PI5P4Ks represent a novel target for disrupting cholesterol homeostasis in p53-deficient cancers.
  • Understanding PI5P4K function provides an upstream mechanism unifying previously observed links to mTOR signaling and tumor proliferation.
  • This research opens new therapeutic strategies by targeting both cholesterol transport and synthesis inhibition.