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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Improving Translational Accuracy02:07

Improving Translational Accuracy

Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...

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Related Experiment Video

Updated: May 24, 2026

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
12:57

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

In vivo single-cell ribosome profiling reveals cell-type-specific translational programs during aging.

Clara Duré1, Umesh Ghoshdastider2, Ramona Weber2

  • 1Institute for Regenerative Medicine (IREM), University of Zurich, Wagistrasse 12, 8952 Schlieren, Zurich, Switzerland; Life Science Zurich Graduate School, Molecular Life Science Program, University of Zurich, ETH Zurich, 8057 Zurich, Switzerland.

Molecular Cell
|May 22, 2026
PubMed
Summary

Aging alters protein synthesis in skin stem cells. This study introduces a new method to map these changes, revealing specific shifts in aged epidermal stem cells that impact their function.

Keywords:
AP-1agingepidermismRNA translationribosome profilingsingle-cell biologytranslational control

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Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Aging Research

Background:

  • Somatic stem cells exhibit low protein synthesis, linked to stemness.
  • The impact of aging on stem cell translational landscapes is largely unknown.

Purpose of the Study:

  • To develop and apply an in vivo single-cell ribosome profiling strategy.
  • To investigate the tissue-wide translational landscapes of the epidermis during aging.
  • To identify cell-type-specific translational reprogramming in aged epidermal stem cells.

Main Methods:

  • Developed an in vivo single-cell ribosome profiling strategy.
  • Utilized ribosomal elongation inhibition and RNase I for enhanced data quality.
  • Analyzed translational landscapes across major epidermal cell types.

Main Results:

  • Documented in vivo translational landscapes of epidermal cells.
  • Identified cell-type-specific translational efficiencies.
  • Discovered significant translational reprogramming of AP-1 subunits in aged epidermal stem cells.

Conclusions:

  • In vivo single-cell ribosome profiling is a powerful tool for mapping cell-type-specific translational programs.
  • Aging induces specific translational reprogramming in epidermal stem cells.
  • The developed strategy offers a scalable approach for tissue-wide translational analysis.