Comparison of CD34-positive cells enumeration using ADAMII image-based fluorescence cell counter with fluorescence...

Area of Science:

• Hematology
• Cell Biology
• Immunology

Background:

• CD34+ cell enumeration is crucial for stem cell transplantation.
• Fluorescence flow cytometry is the gold standard for CD34+ cell counting.
• Image-based methods offer potential alternatives for cell enumeration.

Purpose of the Study:

• To compare CD34+ cell counting between image-based (ADAMII) and fluorescence flow cytometry methods.
• To evaluate the accuracy and reliability of the ADAMII device for CD34+ cell enumeration.

Main Methods:

• Samples from mobilized peripheral blood (MPB) and leukapheresis bags (LB) were analyzed.
• CD34+ cells were enumerated using both flow cytometry (ISHAGE protocol) and the ADAMII device.
• Spearman rank correlation and Bland-Altman analysis were employed to assess agreement and bias.

Main Results:

• A strong positive linear correlation (r_s>0.9, p<0.001) was found between ADAMII and flow cytometry.
• Flow cytometry yielded statistically higher CD34+ cell counts in LB samples.
• No statistically significant difference in counts was observed between methods for MPB samples.

Conclusions:

• The ADAMII device demonstrates strong correlation with flow cytometry for CD34+ cell counting.
• Discrepancies in LB samples suggest potential underestimation by the ADAMII device.
• Further research is needed to confirm ADAMII's clinical utility and avoid potential diagnostic errors.