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Related Experiment Video

Updated: May 24, 2026

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
13:21

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

A scalable Tn5-based method for genome-wide DNA methylation profiling in development and disease.

Hanrong Hu1,2, Nahuel Simonet1,2, Ece Naz Bilgiç1,2

  • 1Ecole Polytechnique Federale de Lausanne (EPFL), School of Life Sciences, Brain Mind Institute, EpiGN-NeuroNA Chair in Epigenomics of Neurodevelopmental Disorders, Station 19, Lausanne, Switzerland.

Nature Communications
|May 22, 2026
PubMed
Summary

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CmeCUT&Tag is a new method for DNA methylation analysis. It offers a cost-effective and scalable approach for epigenetic research and clinical applications, reducing sequencing needs.

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Genomics

Background:

  • DNA methylation is a crucial epigenetic mark regulating gene expression, vital in development and disease, particularly cancer.
  • Existing DNA methylation detection methods like bisulfite sequencing offer base-pair resolution but are limited by high costs and sequencing depth requirements.

Purpose of the Study:

  • To develop a novel, efficient, and cost-effective method for genome-wide DNA methylation profiling.
  • To enable quantitative DNA methylation analysis with reduced input DNA and sequencing demands.

Main Methods:

  • Development of CmeCUT&Tag, a Tn5-based technique utilizing methylation-binding domain fusion proteins.
  • Selective targeting of methylated DNA in both chromatinized and isolated DNA for adapter insertion.

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Related Experiment Videos

Last Updated: May 24, 2026

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
13:21

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

Methyl-binding DNA capture Sequencing for Patient Tissues
08:40

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

  • Facilitation of low-depth sequencing for quantitative analysis or optional cytosine conversion for base-pair resolution.

    • Main Results:

    • CmeCUT&Tag enables genome-wide DNA methylation profiling with significantly reduced input and sequencing requirements.
    • The method demonstrated strong concordance with established bisulfite sequencing techniques.
    • Successful characterization of DNA methylation patterns across various biological contexts, including human stem cells, organoids, zebrafish embryogenesis, and tumor biopsies.
    • Application in classifying brain tumor samples into distinct methylation subtypes.

    Conclusions:

    • CmeCUT&Tag presents a scalable and cost-effective solution for DNA methylation analysis.
    • The method holds promise for advancing epigenetic research and holds potential for clinical diagnostic applications.