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Updated: May 24, 2026

Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells

Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells

Published on: December 13, 2024

Organoids: generation strategies, applications, and future challenges.

Hao Yin^1,2, Guo-Qiang Zhu^1,2, Hai-Zhan Wang³

¹Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.

Stem Cell Research & Therapy

|May 23, 2026

Summary

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Organoid technology uses stem cells to create miniature organ models for research. These 3D organoids offer a more accurate in vitro platform for disease modeling, drug testing, and regenerative medicine.

Area of Science:

Biomedical Research
Stem Cell Biology
Bioengineering

Background:

Organoids are 3D in vitro stem cell-derived models mimicking organ structure and function.
Established in 2009, organoid technology has rapidly advanced for various organs like brain, liver, and kidney.
They offer greater physiological relevance than traditional 2D cultures.

Purpose of the Study:

To review organoid generation strategies and protocols.
To highlight bioengineering innovations enhancing organoid development.
To discuss the broad biomedical applications and challenges of organoid technology.

Main Methods:

Review of scaffold-free and scaffolded organoid generation techniques.
Detailing specific protocols for brain, liver, intestine, kidney, and bone organoids.

Keywords:

3D structure Bioengineering Biological applications Generative strategy Organoids

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Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

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Published on: April 13, 2022

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Last Updated: May 24, 2026

Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells

Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells

Published on: December 13, 2024

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Published on: April 13, 2022

Emphasis on bioengineering integration, including organ-on-a-chip and 3D bioprinting.

Main Results:

Organoids recapitulate tissue heterogeneity, structure, and genetic background.
Bioengineering innovations improve organoid maturity, vascularization, and reproducibility.
Diverse applications demonstrated in disease modeling, drug screening, and regenerative medicine.

Conclusions:

Organoids represent a significant advancement in in vitro research.
Challenges include standardization, vascularization, ethics, and scalability.
Interdisciplinary approaches will drive organoid technology for personalized medicine and regenerative therapies.