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MAP30, a type Ⅰ ribosome-inactivating protein, inhibits hepatocellular carcinoma by inducing apoptosis through

Di Yang1, Di Peng2, Di Jia2

  • 1The Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation 416 Hospital, Chengdu, Sichuan, China.

Medicine
|May 23, 2026
PubMed
Abstract

Insights

Momordica anti-HIV protein of 30 kDa (MAP30) inhibits liver cancer cell proliferation and induces apoptosis. This study explores MAP30's molecular mechanism in liver cancer treatment, revealing its potential as an antitumor drug.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Momordica anti-HIV protein of 30 kDa (MAP30) is a ribosome-inactivating protein with known antiviral, antibacterial, and antitumor activities.
  • MAP30's potential in liver cancer prevention and treatment is speculated but its mechanism remains unclear.

Purpose of the Study:

  • To investigate the molecular mechanism of MAP30's effect on liver cancer cells.
  • To explore MAP30 as a potential therapeutic agent for liver cancer.

Main Methods:

  • Cell Counting Kit-8 assay for proliferation.
  • Flow cytometry for apoptosis, cell cycle, reactive oxygen species (ROS), and mitochondrial membrane potential.
  • Western blot for protein level analysis.

Main Results:

  • MAP30 significantly inhibited SK-Hep-1 liver cancer cell proliferation.
  • MAP30 induced G1 phase cell cycle arrest and apoptosis.
  • Apoptosis involved the mitochondrial pathway, evidenced by increased ROS and decreased mitochondrial membrane potential, and affected key apoptosis-related proteins.

Conclusions:

  • MAP30 demonstrates significant antitumor effects on liver cancer cells.
  • MAP30's mechanism involves inducing apoptosis via the mitochondrial pathway.
  • MAP30 shows promise as a potential antitumor drug for liver cancer treatment.
Keywords:
SK-Hep-1apoptosishepatocellular carcinomamitochondriaribosome-inactivating proteins (RIPs)

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