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Updated: May 25, 2026
Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts
Published on: March 2, 2017
Pregnancy and perinatal outcomes following PIEZO-ICSI with vitrified-warmed single blastocyst transfer: a
Kenichiro Hiraoka1,2, Miki Osugi3, Moe Sato3
1Kameda IVF Clinic Makuhari, Chiba, Japan. ken.msn.hiraoka@gmail.com.
Objective:
To report pregnancy and perinatal outcomes following intracytoplasmic sperm injection using a piezo element (PIEZO-ICSI) in a large Japanese cohort undergoing vitrified-warmed single blastocyst transfer. Outcomes from conventional IVF cycles were included as a reference population sharing similar ovarian stimulation and embryo transfer protocols.
Methods:
This study was a retrospective cohort study conducted in two fertility centers in Japan. A total of 1,822 PIEZO-ICSI cycles from 1,072 patients aged < 40 years at oocyte retrieval were included between June 2016 and August 2023. A reference population of 1,701 IVF cycles from 996 patients was included for context.
Results:
In the PIEZO-ICSI cohort, the clinical pregnancy rate was 49.9% (910/1,822) and the live birth rate was 38.7% (706/1,822). Among live births, the mean birth weight was 3,011.4 ± 467.9 g, mean gestational age was 38.59 ± 1.95 weeks, and congenital anomaly rate was 1.6% (11/706). These outcomes were comparable to the IVF reference population (clinical pregnancy rate 48.4%, live birth rate 37.9%, birth weight 3,022.8 ± 469.5 g, congenital anomaly rate 2.5%). The proportion of female infants was significantly higher in the PIEZO-ICSI group (53.2% vs. 43.4%; P < 0.001).
Conclusion:
PIEZO-ICSI is associated with favorable pregnancy and perinatal outcomes in women aged < 40 years.
