Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of CRISPR/CasRx-Mediated VEGF mRNA Knockdown in Mouse Retina.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Receptor-guided AAV Tropism Engineering via MATCH.

bioRxiv : the preprint server for biology·2026
Same author

Using RNA-targeting CRISPR-Cas13 and engineered U1 systems to target <i>ABCA4</i> splice variants in Stargardt disease.

Molecular therapy. Nucleic acids·2026
Same author

Multiplexed Pan Soluble Ligandome Assaying via OASIS.

bioRxiv : the preprint server for biology·2026
Same author

Regulatable In Vivo Gene Expression via Adaptamers.

bioRxiv : the preprint server for biology·2026
Same author

Genome-wide CRISPR Screening Reveals a PKA-Driven Resistance Mechanism to Metformin for Oral Cancer Prevention That Can Be Exploited by Combination with NSAIDs.

Cancer prevention research (Philadelphia, Pa.)·2025
Same journal

Rational Design of Linalool Dehydratase-Isomerase Enables Efficient Conversion of Phytol to Neophytadiene.

ACS synthetic biology·2026
Same journal

<i>De Novo</i> Biosynthesis of Polyphyllin V in <i>Nicotiana benthamiana</i> through Pathway Reconstruction and UDP-Sugar Engineering.

ACS synthetic biology·2026
Same journal

Rapid and Continuous Directed Evolution in <i>Vibrio natriegens</i> Utilizing an <i>In Vivo</i> Hypermutation System.

ACS synthetic biology·2026
Same journal

Machine Learning for Microbial Cell Factories: Pathway Design, Enzyme Engineering, and Metabolic Regulation.

ACS synthetic biology·2026
Same journal

Microfluidics-Based Engineering of Molecular Self-Assembly and Manufacturing for Artificial Cell Systems.

ACS synthetic biology·2026
Same journal

Beyond Compartmentalization: Deciphering Reaction Kinetics in Liquid-Liquid Phase Separation for Rational Biotechnological Design.

ACS synthetic biology·2026
See all related articles

Related Experiment Video

Updated: May 25, 2026

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants
09:20

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants

Published on: October 18, 2022

Receptor-Guided AAV Tropism Engineering via MATCH.

Nolan Graham1, Satheesh Kumar1, Joseph Rainaldi1,2

  • 1Department of Bioengineering, University of California San Diego, La Jolla, California 92093, United States.

ACS Synthetic Biology
|May 23, 2026
PubMed
Summary
This summary is machine-generated.

We developed MATCH, a novel method for engineering adeno-associated virus (AAV) tropism. This approach allows precise viral retargeting for enhanced gene delivery and therapeutic applications.

Keywords:
AAV capsid engineeringSpyTag/SpyCatcherblood−brain barrierimmune cell engineeringtargeted gene deliverytransferrin receptor (TfR1)

More Related Videos

Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling
21:55

Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol
09:12

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol

Published on: April 5, 2024

Related Experiment Videos

Last Updated: May 25, 2026

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants
09:20

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants

Published on: October 18, 2022

Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling
21:55

Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol
09:12

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol

Published on: April 5, 2024

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Gene Therapy

Background:

  • Controlling viral tropism is crucial for effective gene delivery.
  • Adeno-associated viruses (AAVs) are promising gene therapy vectors but lack precise targeting.
  • Current methods for modifying AAV tropism are limited.

Purpose of the Study:

  • To develop a modular method for programmable retargeting of AAVs.
  • To enable receptor-guided modification of AAV tropism for enhanced gene delivery.
  • To create a scalable and versatile platform for custom AAV vector design.

Main Methods:

  • Developed MATCH (Modulation of AAV Tropism through Conjugation to Homing proteins), a site-specific covalent protein conjugation technique.
  • Incorporated SpyTag peptide into AAV capsid loops for one-step attachment of SpyCatcher-linked targeting proteins.
  • Utilized mosaic AAV-DJ and AAV9 capsids for tunable ligand display and efficient assembly.
  • Established a streamlined one-pot "Mix-and-MATCH" production strategy for coexpression of capsid and targeting ligands.

Main Results:

  • MATCH-AAVs conjugated to anti-CD3 antibodies efficiently transduced human T cells in vitro (up to 58% of PBMCs).
  • TfR1-targeted MATCH-AAV9 vectors showed up to 88-fold increased brain expression in mice.
  • Human TfR1-targeted vectors demonstrated robust, receptor-dependent transduction in vitro and in humanized mouse models.
  • TfR1-targeted vectors facilitated widespread parenchymal transduction, indicating blood-brain barrier crossing.

Conclusions:

  • MATCH provides a versatile synthetic-biology toolkit for rational AAV tropism engineering.
  • This method enables programmable, receptor-guided retargeting of AAVs for research and therapeutic applications.
  • The "Mix-and-MATCH" strategy offers a scalable route to custom AAV vector production.