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Related Experiment Video

Updated: May 25, 2026

Microembossing: A Convenient Process for Fabricating Microchannels on Nanocellulose Paper-Based Microfluidics
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Microembossing: A Convenient Process for Fabricating Microchannels on Nanocellulose Paper-Based Microfluidics

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Nucleic acid detection using paper-based microfluidic chips: From amplification to signal readout.

Jiaxuan Xing1, Chen Li2, Fang Yang1

  • 1Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, 130012, China.

Talanta
|May 23, 2026
PubMed
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Paper-based microfluidics offers a low-cost, portable platform for nucleic acid detection. This review details advances in amplification, signal enhancement, and readout methods for molecular diagnostics.

Area of Science:

  • Microfluidics
  • Nucleic Acid Detection
  • Molecular Diagnostics

Background:

  • Paper-based microfluidics leverages porous paper substrates and capillary flow for nucleic acid analysis.
  • These platforms offer advantages such as low cost, portability, ease of use, and rapid detection.
  • They are a promising tool for scalable molecular diagnostics.

Purpose of the Study:

  • To systematically review recent advancements in paper-based microfluidic nucleic acid detection.
  • To analyze strategies in amplification, signal enhancement, and signal readout.
  • To provide an overview of the field's current state, limitations, and future directions.

Main Methods:

  • Review of amplification strategies including isothermal methods (LAMP, RPA) and CRISPR-assisted systems.
Keywords:
Lab-on-a-chipLiquid biopsyNucleic acid amplificationNucleic acid detectionPaper-based microfluidicsPathogen detection

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Last Updated: May 25, 2026

Microembossing: A Convenient Process for Fabricating Microchannels on Nanocellulose Paper-Based Microfluidics
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Published on: October 6, 2023

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Published on: September 10, 2014

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08:56

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Published on: November 15, 2017

  • Analysis of signal enhancement techniques like catalytic hairpin assembly (CHA), gold nanomaterials, and nanozyme catalysis.
  • Comparative analysis of readout modalities: electrochemical, colorimetric, fluorescence, and SERS.

    • Main Results:

    • Isothermal amplification and CRISPR systems show promise for integration into paper-based platforms.
    • Signal enhancement strategies significantly improve detection sensitivity.
    • Diverse readout methods are adaptable, with ongoing development trends.

    Conclusions:

    • Paper-based microfluidics is a rapidly advancing field for nucleic acid detection with significant potential.
    • Integration of advanced amplification, enhancement, and readout techniques drives performance improvements.
    • Future work should focus on optimization and translational applications for diagnostics.