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Related Experiment Video

Updated: May 25, 2026

Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media
10:46

Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media

Published on: May 13, 2022

Divalent aptamer-mediated clustering for extracellular vesicle separation.

Xiaoling Liu1, Taiyi Zhang1, Qi Zhang1

  • 1Department of Biology, Faculty of Arts and Sciences, Beijing Normal University at Zhuhai, Zhuhai, China.

Communications Biology
|May 23, 2026
PubMed
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A new divalent aptamer-mediated clustering (DAC) method simplifies extracellular vesicle (EV) isolation from various biofluids. This affinity-based approach enhances EV yield and purity, offering a versatile tool for research.

Area of Science:

  • Biotechnology
  • Nanomedicine
  • Molecular Biology

Background:

  • Extracellular vesicles (EVs) are crucial for intercellular communication and serve as biomarker sources.
  • Current EV isolation methods face challenges in balancing yield, purity, and biofluid adaptability.
  • Efficient and reliable EV isolation is critical for diagnostic and therapeutic applications.

Purpose of the Study:

  • To introduce a novel, streamlined affinity-based strategy for extracellular vesicle (EV) isolation.
  • To develop a method that overcomes the limitations of existing EV isolation techniques.
  • To provide a versatile platform for studying EV heterogeneity and function.

Main Methods:

  • Divalent aptamer-mediated clustering (DAC) strategy utilizing multivalent aptamer binding to EV surface markers.

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Related Experiment Videos

Last Updated: May 25, 2026

Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media
10:46

Size Exclusion Chromatography for Separating Extracellular Vesicles from Conditioned Cell Culture Media

Published on: May 13, 2022

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
08:32

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

Purification and Characterization of Extracellular Vesicles from Human Adipose-Derived Mesenchymal Stem Cells
04:54

Purification and Characterization of Extracellular Vesicles from Human Adipose-Derived Mesenchymal Stem Cells

Published on: May 3, 2024

  • Induction of controllable EV clustering to facilitate recovery via standard filtration.
  • Benchmarking DAC against ultracentrifugation, density gradient ultracentrifugation, and size-exclusion chromatography.

    • Main Results:

    • DAC demonstrated robust EV isolation from plasma, urine, and cell culture media.
    • Achieved comparable or improved EV yield and purity with reduced processing time, cost, and complexity.
    • Proteomic and metabolomic analyses revealed distinct cargo profiles of DAC-isolated EV subpopulations.
    • DAC successfully isolated affinity-defined EV subpopulations, including EpCAM-positive EVs.

    Conclusions:

    • DAC offers a versatile, accessible, and efficient platform for extracellular vesicle isolation.
    • The method preserves EV integrity and biological activity, enabling downstream analyses.
    • DAC facilitates the study of EV heterogeneity, function, and molecular composition, advancing biomarker discovery.