Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Variable predictors of acute pulmonary embolism recurrence with duration of follow-up.

Journal of thoracic disease·2020
Same author

Is the result of modified Allen's test still accurate after endoscopic thoracic sympathectomy?

Journal of thoracic disease·2020
Same author

Application of a bilayer tubular scaffold based on electrospun poly(l-lactide-co-caprolactone)/collagen fibers and yarns for tracheal tissue engineering.

Journal of materials chemistry. B·2020
Same author

Isolation of two rare N-glycosides from Ginkgo biloba and their anti-inflammatory activities.

Scientific reports·2020
Same author

Serum Metabolic Profiling Reveals the Antidepressive Effects of the Total Iridoids of <i>Valeriana jatamansi</i> Jones on Chronic Unpredictable Mild Stress Mice.

Frontiers in pharmacology·2020
Same author

Superresolution quantitative imaging based on superoscillatory field.

Optics express·2020

Related Experiment Video

Updated: May 25, 2026

Assay Development for High-Throughput Drug Screening Against Mycobacteria
07:50

Assay Development for High-Throughput Drug Screening Against Mycobacteria

Published on: October 25, 2024

A survey of parallelism testing methods for bioassays.

Chao Wang1, Ji Li2, Chang Chen1

  • 1Office of Biostatistics, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, USA.

Journal of Biopharmaceutical Statistics
|May 24, 2026
PubMed
Summary

This study reviews methods for testing parallelism, a key assumption in bioassays for determining relative potency. Understanding parallelism ensures consistent potency measurements between test and reference products.

Keywords:
Bioassayequivalenceparallelismrelative potencysimilarity

More Related Videos

Assays for the Identification of Novel Antivirals against Bluetongue Virus
12:02

Assays for the Identification of Novel Antivirals against Bluetongue Virus

Published on: October 11, 2013

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

Related Experiment Videos

Last Updated: May 25, 2026

Assay Development for High-Throughput Drug Screening Against Mycobacteria
07:50

Assay Development for High-Throughput Drug Screening Against Mycobacteria

Published on: October 25, 2024

Assays for the Identification of Novel Antivirals against Bluetongue Virus
12:02

Assays for the Identification of Novel Antivirals against Bluetongue Virus

Published on: October 11, 2013

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

Area of Science:

  • Biostatistics
  • Pharmacometrics
  • Assay Development

Background:

  • Parallelism is crucial for defining relative potency between test and reference products in bioassays.
  • It assumes the test product acts as a dilution or concentration of the reference standard.
  • Ensuring parallelism guarantees that relative potency remains constant across different response levels.

Purpose of the Study:

  • To review existing methods for parallelism testing in bioassays.
  • To discuss significance, equivalence, and similarity testing approaches for parallelism.
  • To analyze the advantages and disadvantages of each method, including equivalence margin determination.

Main Methods:

  • Literature review of parallelism testing proposals.
  • Analysis of significance testing approaches.
  • Evaluation of equivalence and similarity testing methods for parallelism.

Main Results:

  • Various methods for parallelism testing exist, each with strengths and weaknesses.
  • Equivalence margin determination is a critical aspect of equivalence testing.
  • Recent advancements offer new approaches to parallelism assessment.

Conclusions:

  • The choice of parallelism testing method impacts the reliability of relative potency estimates.
  • Careful consideration of testing approaches and equivalence margins is necessary.
  • Further research into recent parallelism testing methods is warranted for improved bioassay accuracy.