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Updated: May 26, 2026

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Hemadyne: accordion-inspired perfusion for microphysiological systems.

Ankit Kumar1, Shivanand Pattanshetti1, Rushangi D Patel1

  • 1Department of Biomedical Engineering, College of Engineering, Texas A&M University, College Station, TX, USA.

Nature Communications
|May 24, 2026
PubMed
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This summary is machine-generated.

Hemadyne, a novel mechanical pump, precisely mimics patient blood flow waveforms for microphysiological systems. This technology enhances cell culture longevity and reveals new insights into endothelial cell responses to blood flow dynamics.

Area of Science:

  • Biomedical Engineering
  • Physiology
  • Cell Biology

Background:

  • Existing microphysiological systems (MPS) face limitations in physiological relevance and reproducibility due to inadequate perfusion systems.
  • Current perfusion technologies lack the precision to replicate complex hemodynamic waveforms crucial for accurate in vitro modeling.

Purpose of the Study:

  • To engineer a novel, standalone mechanical pump (Hemadyne) for MPS that overcomes the limitations of existing perfusion systems.
  • To demonstrate Hemadyne's capability in replicating clinical hemodynamic waveforms with high fidelity and sustaining long-term cell culture.

Main Methods:

  • Developed Hemadyne, a compact, instrument-free mechanical pump inspired by accordion physics.
  • Implemented a custom control algorithm for precise waveform generation (400 ms temporal resolution).

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  • Validated Hemadyne's flow replication against Doppler ultrasound waveforms and assessed its performance against conventional systems.

    • Main Results:

    • Hemadyne accurately replicated patient hemodynamic waveforms with high spatiotemporal fidelity.
    • The pump demonstrated superior performance, providing stable flow, transient flows, and fast response times.
    • Hemadyne sustained primary human endothelial cell cultures for up to 60 days in a vessel-chip.
    • The system elucidated the role of the diastolic rest phase in endothelial homeostasis and revealed differential arterial/venous endothelial cell responses.
    • Hemadyne recapitulated age-associated pathological effects of diastolic retrograde flow, which are difficult to model in animals.

    Conclusions:

    • Hemadyne is an enabling technology that significantly enhances the physiological relevance and operational longevity of microphysiological systems.
    • The pump facilitates advanced research into endothelial cell biology and disease modeling under physiologically relevant flow conditions.
    • Hemadyne offers a powerful tool for dissecting complex hemodynamic influences on cellular function and homeostasis.