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Related Experiment Video

Updated: May 26, 2026

Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin
08:52

Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin

Published on: February 3, 2015

Lactic-Acid-Induced Zebrafish Skin Apoptosis as a Model for Evaluating Skin Barrier Function.

Qinqian Tao1, Mingjie He2, Jiaqi Zeng2

  • 1Natuspace Biotechnology Co. Ltd., Guangzhou, China.

Zebrafish
|May 24, 2026
PubMed
Summary
This summary is machine-generated.

This study developed a new zebrafish model to assess skin barrier function. The model effectively evaluates the protective effects of compounds on skin health.

Keywords:
cosmetic ingredientsin vivo modelskin barrierzebrafish inflammation

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Last Updated: May 26, 2026

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Area of Science:

  • Dermatology and Toxicology
  • Zebrafish models in biomedical research

Background:

  • Skin barrier function is vital for skin health.
  • Assessing topical product efficacy requires reliable methods.
  • Existing zebrafish models have limitations for direct skin barrier assessment.

Purpose of the Study:

  • To develop an in vivo, in situ, high-throughput screening model for skin barrier function in zebrafish embryos.
  • To establish a reliable method for evaluating the protective and restorative effects of topical agents on the skin barrier.

Main Methods:

  • Induction of skin barrier damage using lactic acid and assessment via crystal violet staining.
  • Histopathological analysis using H&E, Masson, and Victoria blue staining.
  • Evaluation of cellular apoptosis using in situ acridine orange (AO) staining and flow cytometry with propidium iodide (PI) staining.

Main Results:

  • Lactic acid significantly increased fluorescence and caused epidermal damage and apoptosis in zebrafish embryos.
  • The model demonstrated 35%-45% inhibition with a positive control, indicating sensitivity.
  • Screening of six additional samples yielded promising results, validating the model's application potential.

Conclusions:

  • The lactic-acid-induced zebrafish skin injury model is a reliable in vivo and in situ method.
  • This model offers a high-throughput approach for evaluating skin barrier function.
  • The model is suitable for assessing the efficacy of drugs and compounds on skin barrier health.